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Protocol of PSS4050(v1.7)

protocol of PSS4050, Next Generation Protein Synthesis Kit, in PDF format.
It includes PCR programs for 1st PCR and 2nd PCR as well as transcription and translation method.

Primer List

Lists of 2nd universal primers and tags including FLAG, His, HA, StrepII. This make? no cloning necessary for Next Generation Protein Synthesis Kit

Primer Design Tool for 1st PCR

Excel VBA macro file in zip format. This helps to design 1st gene specific primers. The features are

    • Tm values for forward and reverse primer by GC% method and Nearest Neighbor method
    • You can select forward primer and reverse primer to make the lowest difference of Tm values, from 18 mer overlapped primer to 30 mer overlapped one respectively.