If you are looking for cell-free protein synthesis outside of JAPAN, please check this product (jump to Sigma-Aldrich site)
Packaging |
|
PCR (50 μl x 60)) | |
PCR DNA Polymerase | 60 μl |
10x PCR Buffer | 300 μl |
25 mM MgSO4 | 300 μl |
2 mM dNTP Mix | 300 μl |
Transcription (25 μl x 50) | |
T7 RNA Polymerase | 50 μl |
10x Transcription Buffer | 125 μl |
100 mM DTT | 62.5 μl |
25 mM NTP Mix | 125 μl |
Translation(110ul x 50) | |
Wheat Germ Extract | 1 ml |
Amino Acid Mix | 1 ml |
All-in-One kit comes with high fidelity, high efficency, high speeed DNA polymerase enabling 2 step PCR cycle, in addition to transcription and translation reagent.
The convenience of transcription and traslation remains the same, and PCR can be performed and generate template more accurately and quickly.
For 2 step cycle PCR, you do not need to worry about cycle conditions if the primers are 20 mer or more and the Tm value is 63 ° C * or pver. The PCR time can also be significantly reduced. This kit contains enough PCR enzymes for 60 reactions in 50 ul of PCR. (* Tm value must be calculated by nearest neighbor method)
eg.) 2 step PCR program for 1st gene specific primers
Predenature: 94℃, 2min
(following 30 cycles)
Denature: 98℃, 10 sec
Extension: 68℃, 1 min + 30 sec/kb *
(* if target is less than 1 kbp, 1 min. if target is more than 1 kbp, 1 min + 30 sec/kb duration)
PCR reagents are designed to be used and annealed at 68° C and to be able to eliminating many problems such as non-specific amplification. So you do not need to investigate.
Storage Condition
Wheat Germ Extract, Amino Acid Mix -70℃, Other trascription reagents and PCR reagetts -20℃
Protocol and Primer list
BCF4050 Protocol (in PDF format)
Price, specification and appearance are subject to change without prior notice.
Packaging |
|
Transcription (25 μl x 50) | |
T7 RNA Polymerase | 50 μl |
10x Transcription Buffer | 125 μl |
100 mM DTT | 62.5 μl |
25 mM NTP Mix | 125 μl |
Translation(110ul x 50) | |
Wheat Germ Extract | 1 ml |
Amino Acid Mix | 1 ml |
Kit contains all the necessary reagents for transcription and translation. You can get various active proteins including membrane proteins, growth factors and/or transcription factors regardless of its localization.?
Sufficient amount of reagents was in 6 tubes for 50 reactions at 110 μl, total 5.5 ml sample scale, according to our standard protocol.
Amino Acid Mix contains 20 amino acids in one solution, so there is no worry about precipitation during preparation of the translation solutions.
Storage Condition:
Wheat Germ Extract, Amino Acid Mix -70°C, other regents -20°C
Protocol and Primer List
PSS4050 Protocol(in PDF)
Price, specification and appearance are subject to change without prior notice.
Packaging |
|
Translation(110ul x 50) | |
Wheat Germ Extract | 1 ml |
Amino Acid Mix | 1 ml |
A Set of reagents required for translation, Wheat Germ Extract (1 ml) and Amino Acid Mix (1 ml) for 5.5 ml sample scale.
This can be used in cases where translation is not necessary but only translation is performed , such as using mRNA synthesized in oligo house. For the construct, pelase refer to our protocol.
Storage Condition:
Wheat Germ Extract, Amino Acid Mix -70°C
In addition, for large scale synthesis, we can ship 50 ml or more as bulk. Please ask.
Amino Acid Mix is sold separately, for dialysis, also known as continuous protein synthesis.
Our amino acid mix is 4x and usually 2 ml requires for extradialysate. Thus, AAMX004 is for 4 dialysis reaction.
Storage Condition:
Amino Acid Mix -70°C
Please ask a quote or order at (https://www.funakoshi.co.jp/contents/67843) or the nearest agents to you.