3.1 Translation (Simple Batch)

3.1 Translation (Simple Batch)

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  1. Prepare mRNA solution adjusted to the optimal amount of mRNA per 80 μl.
  2. Prepare the translation reaction solution in a 1.5 ml tube as follows
    Reagents volume
    Wheat Germ Extract 10 μl
    Amino Acid Mix 20 μl
    mRNA solution 80 μl
    total 110 μl
  3. Incubate above 10 hours (to 20 hours) at 16 °C