Required Reagents

Required Reagents

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In addition to our cell-free protein synthesis kit (product number: PSS4050), the necessary reagents are shown for each step of template creation, mRNA synthesis, and protein synthesis. must-to-have items are indicated by ✔✔, and better-to-have items are indicated by ✔

The cell-free protein synthesis all-in-one kit (product number: BCF4050) contains the following PCR enzymes.

Reagent spec template transcription translation
ultra pure water   ✔✔ ✔✔
RNase free water   ✔✔
template DNA 1μg/μℓ ✔✔    
PCR enzymes   ✔✔    
PCR primers   ✔✔    
TAE agarose gel   ✔✔ ✔✔  
ammonium acetate 4M stock   ✔✔  
ethanol 99.5%   ✔✔