template DNA for N-term tagged

template DNA for N-term tagged

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The structure of N-terminal tag template DNA and the sequence of each part are as follows.

Please select one of the table below for the tag sequence. Or prepare with an arbitrary tag.

Linker_N can be changed to an appropriate protease recognition sequence.

Construct

5′ UTR       3’UTR
5′   ATG   STOP 3′
T7 Promoter Enhancer tag
sequence
Linker_N ORF Tail sequence

 

Part / Sequence

Region Site DNA Sequence Tag
5′ UTR T7 Promoter  CCCGCGAAAT TAATACGACT CACTATA  
Enhancer  GGGCTCACCT ATCTCTCTAC ACAAAACATT TCCCTACATA CAACTTTCAACTTCCTATT  
Translated Region Start codon  ATG (or if no-tag, none )  
tag sequence   no-tag
 CAT CAT CAT CAT CAT CAT 6x His
 GAC TAC AAG GAT GAC GAT GAC AAG FLAG
 TAT CCT TAT GAC GTG CCT GAC TAT GCC HA
 TGG TCC CAC CCT CAA TTC GAG AAG strep II
Linker_N  CTC CAG CAG GGA GGT ACT ( Leu-Gln-Gln-Gly-Gly-Thr )  
ORF  ATG + NNNNN ・・・ ・・・NNNNN  
Stop codon  TAA, TAG, TGA  
3′ UTR Tail sequence  AAAAAAAAAA GAGCTCTTGG ATCCGGCCAT AAGGGCCTGATCCTTCGAGG GGGGGCC