2.1 Prepare transcription solution

2.1 Prepare transcription solution

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Prepare the transcription reaction solution in a 1.5 ml tube as follows.
 
 
Do not mix 2nd PCR Product directly with 10x Transcription Buffer.
 
 
Reagents Volume
10x Transcription Buffer 2.5 μl
25 mM NTP Mix  2.5 μl
T7 RNA Polymerase  1 μl
100 mM DTT  1.25 μl
2nd PCR Product  2.5 μl
RNase free water  15.25 μl
total  25 μl