2.2 TranscriptionCreated On2019-08-17bywpmaster You are here: KB Home 3. Protocol 2.2 Transcription < Back Lightly tap the prepared transcription solution, and centrifuge it lightly with a tabletop centrifuge. Cap the tube lid tightly to prevent evaporation of the solution. Incubate with a block heater at 37 °C for 3 hours.