template DNA for N-term tagged
The structure of N-terminal tag template DNA and the sequence of each part are as follows.
Please select one of the table below for the tag sequence. Or prepare with an arbitrary tag.
Linker_N can be changed to an appropriate protease recognition sequence.
Please select one of the table below for the tag sequence. Or prepare with an arbitrary tag.
Linker_N can be changed to an appropriate protease recognition sequence.
Construct
| 5′ UTR | 3’UTR | ||||
| 5′ | ATG | STOP | 3′ | ||
| T7 Promoter | Enhancer | tag sequence |
Linker_N | ORF | Tail sequence |
Part / Sequence
| Region | Site | DNA Sequence | Tag |
| 5′ UTR | T7 Promoter | CCCGCGAAAT TAATACGACT CACTATA | |
| Enhancer | GGGCTCACCT ATCTCTCTAC ACAAAACATT TCCCTACATA CAACTTTCAACTTCCTATT | ||
| Translated Region | Start codon | ATG (or if no-tag, none ) | |
| tag sequence | no-tag | ||
| CAT CAT CAT CAT CAT CAT | 6x His | ||
| GAC TAC AAG GAT GAC GAT GAC AAG | FLAG | ||
| TAT CCT TAT GAC GTG CCT GAC TAT GCC | HA | ||
| TGG TCC CAC CCT CAA TTC GAG AAG | strep II | ||
| Linker_N | CTC CAG CAG GGA GGT ACT ( Leu-Gln-Gln-Gly-Gly-Thr ) | ||
| ORF | ATG + NNNNN ・・・ ・・・NNNNN | ||
| Stop codon | TAA, TAG, TGA | ||
| 3′ UTR | Tail sequence | AAAAAAAAAA GAGCTCTTGG ATCCGGCCAT AAGGGCCTGATCCTTCGAGG GGGGGCC |