Is codon conversion (a.k.a silent mutation) necessary?
Although our translation solution is derived from wheat (Triticum aestivum), codon conversion, also known as silent mutation, does not seem to be necessary. There is a report that reducing the number of rare codon in the coding region of the 50 amino acid residues on the N-terminal is important for translation efficiency *1
In addition, for example, the codon frequency (F / 1000) of CGC for arginine is 19 for humans and 40 for E. coli, so if codon conversion is performed to improve efficiency in E. coli host, there might become rare codon for other host. In this case, early translation termination or frameshifting may occur.
*1 Hiroyuki Imanaka. Codon is the deciding factor! High expression method of recombinant protein. Journal of Biotechnology. 2013. 91. 11. 655-655
In addition, for example, the codon frequency (F / 1000) of CGC for arginine is 19 for humans and 40 for E. coli, so if codon conversion is performed to improve efficiency in E. coli host, there might become rare codon for other host. In this case, early translation termination or frameshifting may occur.
*1 Hiroyuki Imanaka. Codon is the deciding factor! High expression method of recombinant protein. Journal of Biotechnology. 2013. 91. 11. 655-655