Required Reagents
In addition to our cell-free protein synthesis kit (product number: PSS4050), the necessary reagents are shown for each step of template creation, mRNA synthesis, and protein synthesis. must-to-have items are indicated by ✔✔, and better-to-have items are indicated by ✔
The cell-free protein synthesis all-in-one kit (product number: BCF4050) contains the following PCR enzymes.
Reagent | spec | template | transcription | translation |
---|---|---|---|---|
ultra pure water | ✔✔ | ✔ | ✔✔ | |
RNase free water | ✔ | ✔✔ | ✔ | |
template DNA | 1μg/μℓ | ✔✔ | ||
PCR enzymes | ✔✔ | |||
PCR primers | ✔✔ | |||
TAE agarose gel | ✔✔ | ✔✔ | ||
ammonium acetate | 4M stock | ✔✔ | ||
ethanol | 99.5% | ✔✔ |