Peparing primers
When creating a transcription template, it is necessary to make a primer in advance.
Multiple primers are required for two-step PCR. The primer list attached to the protocol shows multiple different tag sequences and lists the forward and reverse primers when attaching each tag to the N-terminus or C-terminus.
Multiple primers are required for two-step PCR. The primer list attached to the protocol shows multiple different tag sequences and lists the forward and reverse primers when attaching each tag to the N-terminus or C-terminus.
Primers listed as 1st_ORF-XX are ORF-specific primers, and those listed as 2nd-XXX are universal primers. The universal primer is designed to be used with other ORFs, which makes it possible to efficiently create transcription templates containing multiple tag sequences.