3.1 Translation (Simple Batch)
- Prepare mRNA solution adjusted to the optimal amount of mRNA per 80 μl.
- Prepare the translation reaction solution in a 1.5 ml tube as follows
Reagents volume Wheat Germ Extract 10 μl Amino Acid Mix 20 μl mRNA solution 80 μl total 110 μl
- Incubate above 10 hours (to 20 hours) at 16 °C