2.3 Purify mRNA

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2.3 Purify mRNA

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  1. After transcription reaction, add 10 μl of 4M ammonium acetate to the 25 μl solution and mix.
  2. Add 100 μl of 100 % ethanol and mix.
  3. centrifuge lightly with a tabletop centrifuge and leave at -20 °C to -80°C for 10 minutes.
  4. Centrifuge at 12000 rpm, 4 °C for 15 minutes.
  5. After removing the supernatant, lightly flush and remove the supernatant again.
  6. Leave untile the precipitate is semi dry with keeping lid open. Be careful not to get any impurities in.
  7. Add 40 μl RNase free water to dissolve the mRNA pellet.