template DNA for C-term tagged

template DNA for C-term tagged

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The construct of C-terminal tag template DNA and the sequence of each part are as follows.

Please select one of the table below for the tag sequence. Or prepare with an arbitrary tag.

Linker_C can be changed to an appropriate protease recognition sequence.

Construct

5′ UTR       3’UTR
5′   ATG   STOP 3′
T7 Promoter Enhancer ORF Linker_C tag
sequence
Tail sequence

 

Part / Sequence

Region Site DNA Sequence Tag
5′ UTR T7 Promoter  CCCGCGAAAT TAATACGACT CACTATA  
Enhancer  GGGCTCACCT ATCTCTCTAC ACAAAACATT TCCCTACATA CAACTTTCAACTTCCTATT  
Translated Region ORF  ATG + NNNNN ・・・ ・・・NNNNN  
Linker_C  GGT CTC CAG CAG GGA GGT ACT ( Gly-Leu-Gln-Gln-Gly-Gly-Thr )  
tag sequence  CAT CAT CAT CAT CAT CAT 6x His
 GAC TAC AAG GAT GAC GAT GAC AAG FLAG
 TAT CCT TAT GAC GTG CCT GAC TAT GCC HA
 TGG TCC CAC CCT CAA TTC GAG AAG strep II
Stop codon  TAA, TAG, TGA  
3′ UTR Tail sequence  AAAAAAAAAA GAGCTCTTGG ATCCGGCCAT AAGGGCCTGATCCTTCGAGG GGGGGCC